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Inhibitory effect of polyphenolic-rich extract from Cola nitida (Kolanut) seed on key enzyme linked to type 2 diabetes and Fe2+ induced lipid peroxidation in rat pancreas in vitro

Identifieur interne : 000A29 ( Main/Exploration ); précédent : 000A28; suivant : 000A30

Inhibitory effect of polyphenolic-rich extract from Cola nitida (Kolanut) seed on key enzyme linked to type 2 diabetes and Fe2+ induced lipid peroxidation in rat pancreas in vitro

Auteurs : Ganiyu Oboh [Nigeria] ; Kate E. Nwokocha [Nigeria] ; Ayodele J. Akinyemi [Nigeria] ; Adedayo O. Ademiluyi [Nigeria]

Source :

Asian Pacific Journal of Tropical Biomedicine [ 2221-1691 ] ; 2014.

RBID : PMC:4025302

Abstract

Objective

To investigate the inhibitory effect of phenolic-rich extracts from Cola nitida (C. nitida) seeds on key enzymes linked with type-2 diabetes and Fe²⁺ induced oxidative stress in rat pancreas.

Methods

The phenolic extract was prepared with 80% acetone (v/v). Subsequently, the antioxidant properties and inhibitory effect of the extract on α - amylase and α - glucosidase as well as on Fe²⁺ induced lipid peroxidation in rat pancreas were determined in vitro.

Results

The result revealed that C. nitida extract inhibited α-amylase (EC₅₀=0.34 mg/mL) and α-glucosidase (EC₅₀=0.32 mg/mL) activities as well as Fe²⁺ induced lipid peroxidation in rat pancreas in a dose dependent manner. In addition, the extract had high DPPH radical scavenging ability (EC₅₀=2.2 mg/mL) and reducing power (8.2 mg AAE/g). Characterization of the main phenolic compounds of the extract using gas chromatography analysis revealed catechin (6.6 mg/100 g), epicatechin (3.6 mg/100 g), apigenin (5.1 mg/100 g) and naringenin (3.6 mg/100 g) were the main compounds in the extract.

Conclusions

This antioxidant and enzyme inhibition could be some of the possible mechanism by which C. nitida is use in folklore for the management/treatment of type-2 diabetes. However, the enzyme inhibitory properties of the extract could be attributed to the presence of catechin, epicatechin, apigenin and naringenin.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4025302

DOI: 10.12980/APJTB.4.2014C75
PubMed: 25183118
PubMed Central: 4025302

Affiliations:

Le document en format XML

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 induced lipid peroxidation in rat pancreas <italic>in vitro</italic>
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<affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Biochemistry, Federal University of Technology, P.M.B., 704, Akure 340001, Nigeria</addr-line>
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<author><name sortKey="Nwokocha, Kate E" sort="Nwokocha, Kate E" uniqKey="Nwokocha K" first="Kate E." last="Nwokocha">Kate E. Nwokocha</name>
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<affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Biochemistry, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria</addr-line>
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<author><name sortKey="Ademiluyi, Adedayo O" sort="Ademiluyi, Adedayo O" uniqKey="Ademiluyi A" first="Adedayo O." last="Ademiluyi">Adedayo O. Ademiluyi</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Inhibitory effect of polyphenolic-rich extract from <italic>Cola nitida</italic>
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 induced lipid peroxidation in rat pancreas <italic>in vitro</italic>
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<author><name sortKey="Nwokocha, Kate E" sort="Nwokocha, Kate E" uniqKey="Nwokocha K" first="Kate E." last="Nwokocha">Kate E. Nwokocha</name>
<affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Department of Biochemistry, University of Ibadan, Ibadan, Nigeria</addr-line>
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<wicri:regionArea>Department of Biochemistry, University of Ibadan, Ibadan</wicri:regionArea>
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<front><div type="abstract" xml:lang="en"><sec><title>Objective</title>
<p>To investigate the inhibitory effect of phenolic-rich extracts from <italic>Cola nitida</italic>
 (<italic>C. nitida</italic>
) seeds on key enzymes linked with type-2 diabetes and Fe<sup>2+</sup>
 induced oxidative stress in rat pancreas.</p>
</sec>
<sec><title>Methods</title>
<p>The phenolic extract was prepared with 80% acetone (v/v). Subsequently, the antioxidant properties and inhibitory effect of the extract on α - amylase and α - glucosidase as well as on Fe<sup>2+</sup>
 induced lipid peroxidation in rat pancreas were determined <italic>in vitro</italic>
.</p>
</sec>
<sec><title>Results</title>
<p>The result revealed that <italic>C. nitida</italic>
 extract inhibited α-amylase (EC<sub>50</sub>
=0.34 mg/mL) and α-glucosidase (EC<sub>50</sub>
=0.32 mg/mL) activities as well as Fe<sup>2+</sup>
 induced lipid peroxidation in rat pancreas in a dose dependent manner. In addition, the extract had high DPPH radical scavenging ability (EC<sub>50</sub>
=2.2 mg/mL) and reducing power (8.2 mg AAE/g). Characterization of the main phenolic compounds of the extract using gas chromatography analysis revealed catechin (6.6 mg/100 g), epicatechin (3.6 mg/100 g), apigenin (5.1 mg/100 g) and naringenin (3.6 mg/100 g) were the main compounds in the extract.</p>
</sec>
<sec><title>Conclusions</title>
<p>This antioxidant and enzyme inhibition could be some of the possible mechanism by which <italic>C. nitida</italic>
 is use in folklore for the management/treatment of type-2 diabetes. However, the enzyme inhibitory properties of the extract could be attributed to the presence of catechin, epicatechin, apigenin and naringenin.</p>
</sec>
</div>
</front>
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<affiliations><list><country><li>Nigeria</li>
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<region><li>État d'Oyo</li>
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<settlement><li>Ibadan</li>
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<orgName><li>Université d'Ibadan</li>
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<tree><country name="Nigeria"><noRegion><name sortKey="Oboh, Ganiyu" sort="Oboh, Ganiyu" uniqKey="Oboh G" first="Ganiyu" last="Oboh">Ganiyu Oboh</name>
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<name sortKey="Ademiluyi, Adedayo O" sort="Ademiluyi, Adedayo O" uniqKey="Ademiluyi A" first="Adedayo O." last="Ademiluyi">Adedayo O. Ademiluyi</name>
<name sortKey="Akinyemi, Ayodele J" sort="Akinyemi, Ayodele J" uniqKey="Akinyemi A" first="Ayodele J." last="Akinyemi">Ayodele J. Akinyemi</name>
<name sortKey="Akinyemi, Ayodele J" sort="Akinyemi, Ayodele J" uniqKey="Akinyemi A" first="Ayodele J." last="Akinyemi">Ayodele J. Akinyemi</name>
<name sortKey="Nwokocha, Kate E" sort="Nwokocha, Kate E" uniqKey="Nwokocha K" first="Kate E." last="Nwokocha">Kate E. Nwokocha</name>
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Inhibitory effect of polyphenolic-rich extract from Cola nitida (Kolanut) seed on key enzyme linked to type 2 diabetes and Fe2+ induced lipid peroxidation in rat pancreas in vitro

Inhibitory effect of polyphenolic-rich extract from Cola nitida (Kolanut) seed on key enzyme linked to type 2 diabetes and Fe2+ induced lipid peroxidation in rat pancreas in vitro

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Abstract

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